Beclin-1 Mouse Monoclonal Antibody (5C2)

Mouse Anti-Beclin-1 Mouse Monoclonal Antibody (5C2)

Product Specifications

Background

BECN1 (beclin 1) encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

BECN1

Gene ID

8678

Accession Number

Q14457

Reactivity

Human, Rat, Mouse, Bovine

Immunogen

Synthetic Peptide of Beclin-1 at AA range of 110-190

Clonality

Monoclonal

Tag

BECN1

Applications

WB, IHC, IF

Purification Method

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Molecular Weight

60kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Beclin-1 protein detects endogenous levels of BECN1.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000; IHC 1:100-200; IF 1:50-200