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IPKine™ HRP, Goat Anti-Mouse IgG HCS

IPKine™ HRP Goat Anti-Mouse IgG heavy chain specific secondary antibody

Product Specifications

Background

Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.

CAS Number

9007-83-4

Reactivity

Mouse

Immunogen

Mouse IgG Fc Fragment

Clonality

Polyclonal

Conjugation

HRP

Tag

Goat Anti-Mouse IgG

Type

Sample Preparation and detection

Applications

WB-IP

Purification Method

Affinity purified using solid phase Mouse IgG (H+L) Fc Fragment with finally > 95% purity based on SDS-PAGE

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination) . Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy) . Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots) .

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody reacts with Fc portion of mouse IgG heavy chain, while it doesn’t react with the Fab portion of mouse IgG. It's cross-reaction with human, bovine and horse serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins

Formulation

Liquid solution

Buffer pH

Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:10000), IP: 1:1,000-1:10,000 (1:2000 optimal)

Tested Applications

WB#IP

Host or Source

Goat

Isotype

Goat IgG

Available Sizes

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