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PurKine™ Biotin-Tag Streptavidin Resin 6FF

PurKine™ Biotin-Tag Protein Purification Streptavidin Resin 6FF

Product Specifications

Background

PurKine™ Biotin-Tag Purification system is based on innovative high-capacity matrix for convenient single-step purification of biotin and biotinylated substances. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Biotin-Tag Purification resin is also available as prepacked spin column and kit formats.

Product Name Alternative

Streptavidin ; Biotin resin; Streptavidin resin; Biotin tag resin

Tag

Biotin Tag

Type

Sample Preparation and detection

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

• High capacity—1 to 3 mg of biotinylated BSA per mL of resin.More than 120 nmol Biotin per mL of resin • High performance—Streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding. • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits.• Robust—highly crosslinked beads tolerate linear flow rates up to 500 cm/hour.

Shipping Conditions

Blue ice

Storage Conditions

Stable for one year at 2-8°C from date of shipment. Do not freeze.

Formulation

Liquid solution with 50% slurry in 20% ethanol.

Buffer pH

Liquid in PBS buffer containing 20% ethanol.

Applications Notes

PurKine™ Biotin-Tag Streptavidin Packed Column effectively purifies high levels of biotin and biotinylated substances in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of cross-linked 6% agarose, to which Streptavidin has been coupled. Tests confirm that performance equals or exceeds popular Streptavidin resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up.

Other Product Names

Streptavidin ; Biotin resin; Streptavidin resin; Biotin tag resin

Tested Applications

WB#IP

Available Sizes

Frequently Asked Questions

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