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PurKine™ GST-Tag Glutathione Resin

PurKine™ GST Tagged Protein Purification Glutathione Resin

Product Specifications

Background

PurKine™ GST-Tag Purification system is based on innovative high-capacity matrix for convenient single-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ GST-Tag Purification resin is also available as prepacked spin column and kit formats.

Product Name Alternative

Glutathione; GST resin; Glutathione resin; GST tag resin

Tag

GST Tag

Type

Sample Preparation and detection

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

• High capacity—More than 20 mg of GST-tagged protein per milliliter of resin. • High performance—Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality. • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits.

Shipping Conditions

Blue ice

Storage Conditions

Stable for one year at 2-8°C from date of shipment. Do not freeze.

Formulation

Liquid solution with 50% slurry in 20% ethanol.

Buffer pH

Liquid in PBS buffer containing 20% ethanol.

Applications Notes

PurKine™ GST-Tag Glutathione Resin effectively purifies high levels of overexpressed GST-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of cross-linked 4% agarose, to which reduced glutathione has been coupled. Tests confirm that performance equals or exceeds popular GST-Tag Purification Resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

Other Product Names

Glutathione; GST resin; Glutathione resin; GST tag resin

Tested Applications

WB#IP

Available Sizes

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