ExKine™ Nuclear Protein Extraction Kit

The preparation of an extract from nuclei is often the first step in studying nuclear proteins and their interactions. The resulting preparation can be used directly in the Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations. • Fast and convenient—non-denatured, active proteins are purified in less than two hours. • Compatible—apply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.

Abbkine ExKine™ Nuclear Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.