Calpain 1 Rabbit Polyclonal Antibody
Calpain 1, also designated ?-calpain, is an intracellular calcium-dependent protease that cleaves cyto-skeletal and submembranous proteins. Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
CANP 1, muCANP, CAPN1, CANPL1, PIG30
Swiss Prot
P07384 Human, O35350 Mouse, P97571 Rat
Cellular Locus
Cytoplasm. Cell membrane.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within Human Calpain 1 aa 293-519 / 714.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
HUVEC, MCF-7, human tonsil tissue, human kidney tissue, 2 kidney tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 82 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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