Restriction enzyme FokI (Internal) Rabbit Polyclonal Antibody
The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
Product Specifications
CAS Number
9007-83-4
Abbreviation
R.FokI
Swiss Prot
P14870 Planomicrobium okeanokoites
Host
Rabbit
Species Reactivity
Planomicrobium okeanokoites
Immunogen
Synthetic peptide within Planomicrobium okeanokoites fokIR aa 444-456.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB
Positive Control
Purified restriction enzyme FokI
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 66 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CD44v9 (Marker of Tumor Metastasis) (rCD44v9/1459), Biotin conjugate, 0.1mg/mL
