T7 tag Rabbit Polyclonal Antibody
The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. This antibody recognizes the T7-tag fused to either the amino- or carboxy-terminus of targeted proteins.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
10 antibody Major capsid protein 10A antibody Major capsid protein antibody Minor capsid protein 10B antibody T7 epitope tag antibody
Host
Rabbit
Species Reactivity
Universal
Immunogen
Recombinant protein fused with T7 Tag.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, ELISA
Positive Control
T7 tagged fusion protein
Concentration
1 mg/mL
Dilution
WB: 1:2,000-1:5,000 ;ELISA: 1:10,000
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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