HMGB1 Rabbit Polyclonal Antibody
Like the histones, HMGB1, also known as high-mobility group protein 1 (HMG-1) is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 is secreted by immune cells (like macrophages, monocytes and dendritic cells) through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. In recent research, HMGB1 has been reported as a novel biomarker for human ovarian cancer.
Product Specifications
Product Name Alternative
Abbreviation
HMG-1
Swiss Prot
P09429 Human
Cellular Locus
Nucleus, Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum, Endosome, Membrane, Secreted.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within N-terminal human HMGB1.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P, FC
Positive Control
Wild-type Raw264.7 whole cell lysate, MCF-7 cell lysate, PC12 cell lysate, F9 cell lysate, Hela, mouse spleen tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000;IF-Cell: 1:200-1:500;IHC-P: 1:200;FC: 1:100
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 25 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
CAS Number
9007-83-4
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