Vimentin Rabbit Polyclonal Antibody
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two. Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody
Abbreviation
VIM
Swiss Prot
P08670 Human
Cellular Locus
Cytoplasm, Cell membrane, Cytoskeleton, Intermediate filament, Membrane, Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within residues of Human Vimentin aa 417-466 / 466.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
Hela cell lysate, A549 cell lysate, 293T cell lysate, Hela, human breast carcinoma tissue, Wild-type Hela whole cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000;IF-Cell: 1:100-1:200;IHC-P: 1:200
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 54 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
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