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Vimentin Rabbit Polyclonal Antibody

Selected Variant

R1308-6

Size: 100 µL

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two. Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways.

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody

Abbreviation

VIM

Swiss Prot

P08670 Human

Cellular Locus

Cytoplasm, Cell membrane, Cytoskeleton, Intermediate filament, Membrane, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within residues of Human Vimentin aa 417-466 / 466.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

Hela cell lysate, A549 cell lysate, 293T cell lysate, Hela, human breast carcinoma tissue, Wild-type Hela whole cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000;IF-Cell: 1:100-1:200;IHC-P: 1:200

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 54 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No

Price

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