Nucleolin Rabbit Polyclonal Antibody
Nucleolin is a protein that in humans is encoded by the NCL gene. Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Nucleolin is also able to act as a transcriptional coactivator with Chicken Ovalbumin Upstream Promoter Transcription Factor II (COUP-TFII).
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
C23 antibody FLJ45706 antibody MS1116 antibody NCL antibody Nucl antibody NUCL_HUMAN antibody Nucleolin antibody Protein C23 antibody
Abbreviation
NCL
Swiss Prot
P19338 Human
Cellular Locus
Nucleus, nucleolus, Cytoplasm.
Host
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthetic peptide within Human Nucleolin aa 201-250 / 710.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell
Positive Control
HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, A431 cell lysate, HepG2 cell lysate, A549 cell lysate, mouse embryonic stem.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:100-1:200
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 77 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
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