Stella Rabbit Polyclonal Antibody
Stella is a primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. It participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. It is important for the totipotent/pluripotent states continuing through preimplantation development.
Product Specifications
Product Name Alternative
Abbreviation
DPPA3, STELLAR
Swiss Prot
Q6W0C5 Human,
Cellular Locus
Cell nucleus, cytoplasm
Host
Rabbit
Species Reactivity
Human,Mouse,Zebrafish
Immunogen
Synthetic peptide within mouse Stella aa 101-150 / 150 (ID:Q8QZY).
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IHC-P
Positive Control
HeLa cell lysate, HEK-293 cell lysate, F9 cell lysate, NCCIT cell lysate, mouse teratoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000 ;IHC-P: 1:100-1:200
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 18 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
CAS Number
9007-83-4
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