TNF alpha Rabbit Polyclonal Antibody
TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells and macrophages. TNF-α plays a key regulatory role in infammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. The antibody detects active recombinant murine TNF alpha. The detective sensitivity by Western Blot is 5ng.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Swiss Prot
P06804 Mouse
Cellular Locus
Secreted, membrane, cytoplasm.
Host
Rabbit
Species Reactivity
Mouse
Immunogen
Synthetic peptide within mouse TNF alpha aa 40-80 and aa 170-210.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, ELISA
Positive Control
Recombinant murine TNF alpha
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000 ;ELISA: 1:10,000-20,000
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 26 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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