SMCC Mouse Monoclonal Antibody [6-F6]
Succinimidyl-4- (N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive N -hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of SMCC and Sulfo-SMCC and are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Succinimidyl-4- (N-maleimidomethyl)cyclohexane-1-carboxylate antibody
Host
Mouse
Species Reactivity
Universal
Immunogen
Recombinant protein fused with SMCC.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
ELISA
Concentration
2 mg/mL
Dilution
ELISA: 1:50,000-1:100,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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