Alpha-2-macroglobulin Mouse Monoclonal Antibody [A5-A9]
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
Alpha-2-M
Swiss Prot
P01023 Human
Cellular Locus
Secreted.
Host
Mouse
Species Reactivity
Human
Immunogen
Recombinant protein within human Alpha-2-macroglobulin aa 950-1200.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, IHC-P, ELISA
Positive Control
LOVO cell lysates, LO2 cell lysates, Hela, HepG2, human liver tissue, human uterus tissue.
Concentration
2 mg/mL
Dilution
WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:100-1:500 ;ELISA: 1:1,000-1:10,000
Purity
Protein G affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 163 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
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