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Histone H3 Mouse Monoclonal Antibody [A11-D7]

The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

H3 histone family member E pseudogene antibody H3 histone family member A antibody H3/A antibody H31_HUMAN antibody H3F3 antibody H3FA antibody Hist1h3a antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody histone 1 H3a antibody Histone cluster 1 H3a antibody Histone H3 3 pseudogene antibody Histone H3.1 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody

Abbreviation

HIST1H3A

Swiss Prot

P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P68433 Mouse, P84228 Mouse, Q6LED0 Rat

Cellular Locus

Nucleus.

Host

Mouse

Species Reactivity

Human,Mouse,Rat,Zebrafish

Immunogen

Synthetic peptide within N-terminal human Histone H3.

Isotype

IgG2b

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IF-Tissue

Positive Control

HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, HEK-293 cell lysate, C2C12 cell lysate, L-929 cell lysate, C6 cell lysate, zebrafish tissue lysates, HepG2 cell lysate, A431 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Hela, F9, human skin tissue, human liver tissue, human testis tissue, mouse brain tissue, mouse testis tissue, rat testis tissue.

Concentration

2 mg/mL

Dilution

WB: 1:5,000-1:10,000 ;IF-Cell: 1:200-1:500 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No

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