6X His tag Mouse Monoclonal Antibody [8-F0]
A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
6 His epitope tag antibody Hexa His tag antibody HHHHHH epitope tag antibody HHHHHH tag antibody His tag antibody Polyhistidine Tag antibody
Host
Mouse
Species Reactivity
Universal
Immunogen
Synthetic peptide immune sequence is CHHHHHHHH.
Isotype
IgG2a
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IP
Positive Control
C-terminal His-tagged recombinant protein.
Concentration
2 mg/mL
Dilution
WB: 1:2,000 ;IP: 2-5 µg/ml.
Purity
Protein G affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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