Histone H4 (acetyl K8) Recombinant Rabbit Monoclonal Antibody [PS01-25]
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H4K8ac is part of 17 modifications of a group of active promoters. H4K8ac is found more often in active promoters and transcribed regions than other marks. H4K8ac is modified by a different group of enzymes than other H4 lysines.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Histone H4 H4C1 H4/A, H4FA, HIST1H4A H4C2 H4/I, H4FI, HIST1H4B H4C3 H4/G, H4FG, HIST1H4C H4C4 H4/B, H4FB, HIST1H4D H4C5 H4/J, H4FJ, HIST1H4E H4K8ac
Swiss Prot
P62805 Human, P62806 Mouse, P62804 Rat
Cellular Locus
Nucleus. Chromosome.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human Histone H4 aa 1-20 (acetyl K8).
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IHC-P, ChIP
Positive Control
Human skin tissue, rat skin tissue, human colon carcinoma tissue, PC-12, Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:50 ;IHC-P 1:1,000-1:5,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 11 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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