PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]
Bifunctional polynucleotide phosphatase/kinase is an enzyme that in humans is encoded by the PNKP gene. A detailed structural study of the crystallized mouse protein examined both the 5´-polynucleotide kinase and 3’-polynucleotide phosphatase activities. Additional features of the peptide sequence include a forkhead association (FHA) domain, ATP binding site and nuclear and mitochondrial localization sequences. PNKP has been shown to interact with DNA polymerase beta and XRCC1. The human gene encoding PNKP was observed to be mutated in patients with microcephaly, seizures and defects in DNA repair. A type of recessive ataxia is also associated with PNKP mutations. There are also newly characterized pathological variants of PNKP. Model organisms such as mice and Drosophila have been used to generate further insights.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
PNKP
Swiss Prot
Q96T60 Human
Cellular Locus
Nucleus.
Host
Rabbit
Species Reactivity
Human
Immunogen
Synthetic peptide within human PNKP aa 460-509/521.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P
Positive Control
HepG2 cell lysate, 293 cell lysate, PC-3 cell lysate, Jurkat cell lysate, human colon carcinoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500 ;IHC-P: 1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 57 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
