iFluor™ 647 Conjugated Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody [ST0533]
The sodium–potassium pump (sodium–potassium adenosine triphosphatase, also known as Na⁺/K⁺-ATPase, Na⁺/K⁺ pump, or sodium–potassium ATPase) is an enzyme (an electrogenic transmembrane ATPase) found in the membrane of all animal cells. It performs several functions in cell physiology. The Na⁺/K⁺-ATPase helps maintain resting potential, affects transport, and regulates cellular volume. It also functions as a signal transducer/integrator to regulate the MAPK pathway, reactive oxygen species (ROS), as well as intracellular calcium. In fact, all cells expend a large fraction of the ATP they produce (typically 30% and up to 70% in nerve cells) to maintain their required cytosolic Na and K concentrations. For neurons, the Na⁺/K⁺-ATPase can be responsible for up to 3/4 of the cell's energy expenditure. In many types of tissue, ATP consumption by the Na⁺/K⁺-ATPases have been related to glycolysis. This was first discovered in red blood cells (Schrier, 1966), but has later been evidenced in renal cells, smooth muscles surrounding the blood vessels,[6] and cardiac purkinje cells. Recently, glycolysis has also been shown to be of particular importance for Na⁺/K⁺-ATPases in skeletal muscles, where inhibition of glycogen breakdown (a substrate for glycolysis) leads to reduced Na⁺/K⁺-ATPase activity and lower force production.
Product Specifications
Product Name Alternative
Abbreviation
Na (+)/K (+) ATPase alpha-1 subunit, ATP1A1, ATP1B1, ATP1B, Na (+)/K (+) ATPase alpha-4 subunit, ATP1A4, ATP1AL2
Swiss Prot
P05023 Human, P05026 Human, Q13733 Human, P06685 Rat, P07340 Rat, Q64541 Rat
Cellular Locus
Cell membrane, Melanosome.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human ATP1A1 aa 39-83 / 1023.
Isotype
IgG
Conjugation
iFluor™ 647
Type
Recombinant Rabbit monoclonal Antibody
Applications
IF-Tissue, FC
Positive Control
Rat kidney tissue, human liver tissue, Hela.
Concentration
1 mg/mL
Dilution
IF-Tissue: 1:100;FC: 1:500-1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Molecular Weight
100 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
CAS Number
9000-83-3
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