CENPB Mouse Monoclonal Antibody [A5F5]
This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
CENPB
Swiss Prot
P07199 Human
Cellular Locus
Nucleus, centromere.
Host
Mouse
Species Reactivity
Human
Immunogen
Recombinant protein within human CENPB aa 1-200 / 599.
Isotype
IgG2a
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, IHC-P, IP
Positive Control
Hela cell lysate, A549 cell lysate, MCF-7 cell lysate, SK-OV-3, A549, human kidney tissue, human skin tissue.
Concentration
2 mg/mL
Dilution
WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:400 ;IHC-P: 1:100-1:500 ;IP: Use at an assay dependent concentration.
Purity
Protein G affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
65 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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