RUNX3 Mouse Monoclonal Antibody [A7B7]
Runt-related transcription factor 3 is a protein that in humans is encoded by the RUNX3 gene. This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. In melanocytic cells RUNX3 gene expression may be regulated by MITF. Most of this literature used RUNX3 promoter methylation status in various cancers as a proxy for its expression. However, quite many genes are known to be methylated in tumor cell genomes, and the majority of these genes are not expressed in the normal tissue of origin of these cancers.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
RUNX3
Swiss Prot
Q13761 Human, Q91ZK1 Rat
Cellular Locus
Cytoplasm, Nucleus.
Host
Mouse
Species Reactivity
Human,Rat
Immunogen
Recombinant protein within human RUNX3 aa 151-350/415.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IHC-P
Positive Control
SW480 cell lysates, Raji cell lysates, rat large intestine tissue, human tonsils tissue, human colon tissue.
Concentration
2 mg/mL
Dilution
WB: 1:500-1:2,000 ;IHC-P: 1:200-1:600
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 44 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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