p47-phox Mouse Monoclonal Antibody [A6B10]
The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47phox and p67-phox. The cytosolic proteins p47phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47phox, suggesting that p47phox may faciliate the transport of p67-phox to the membrane.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
NCF1
Swiss Prot
P14598 Human, Q09014 Mouse, F1M707 Rat
Cellular Locus
Cytosol, Membrane.
Host
Mouse
Species Reactivity
Human,Mouse
Immunogen
Recombinant protein within human p47-phox aa 51-250.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, IHC-P, FC
Positive Control
Raji cell lysates, Daudi cell lysates, Hela, NIH/3T3, human lung tissue, human spleen tissue, Daudi.
Concentration
2 mg/mL
Dilution
WB: 1:2,000-1:5,000;IF-Cell: 1:50-1:200;IHC-P: 1:1,000-1:2,000;FC: 1:100
Purity
Protein G affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
45 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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