NSUN4 Mouse Monoclonal Antibody [4G4]
Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
5-methylcytosine rRNA methyltransferase NSUN4 NOL1/NOP2/Sun domain family member 4
Swiss Prot
Q96CB9 Human, Q9CZ57 Mouse
Cellular Locus
Mitochondrion.
Host
Mouse
Species Reactivity
Human,Mouse
Immunogen
Recombinant protein within human NSUN4 aa 1-384.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IHC-P
Positive Control
MCF-7 cell lysate, 293 cell lysate, mouse testis tissue, human prostate tissue.
Concentration
2 mg/mL
Dilution
WB: 1:500-1:1,000;IHC-P: 1:100-1:500
Purity
Protein G affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
43 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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