MED12 Rabbit Polyclonal Antibody
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
Swiss Prot
Q93074 Human
Cellular Locus
Nucleus.
Host
Rabbit
Species Reactivity
Human
Immunogen
Recombinant protein within human MED12 aa 1250-1450.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IHC-P
Positive Control
Hela cell lysate, K562 cell lysate, human colon carcinoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000 ;IHC-P: 1:200
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 243 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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