BRG1 Rabbit Polyclonal Antibody
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
Swiss Prot
P51532 Human, Q3TKT4 Mouse
Cellular Locus
Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthetic peptide within human BRG1 aa 200-300 / 1647.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IHC-P
Positive Control
293 cell lysate, Daudi cell lysate, mouse testis tissue, human lung carcinoma tissue, Wild-type HepG2 whole cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000;IHC-P: 1:50-1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
181/187 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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