Granzyme B Rabbit Polyclonal Antibody
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse . It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death . Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis .
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
Swiss Prot
P10144 Human
Cellular Locus
Secreted.
Host
Rabbit
Species Reactivity
Human
Immunogen
Recombinant protein within human Granzyme B aa 50-247.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
IHC-P, mIHC
Positive Control
human spleen tissue, human cervical cancer.
Concentration
1 mg/mL
Dilution
IHC-P: 1:400 ;mIHC: 1:200
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 28 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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