ERAB Rabbit Polyclonal Antibody
In addition to mitochondrial dehydrogenase activity, moonlights as a component of mitochondrial ribonuclease P, a complex that cleaves tRNA molecules in their 5'-ends . Together with TRMT10C/MRPP1, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity . The MRPP1-MRPP2 subcomplex catalyzes the formation of N1-methylguanine and N1-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs; HSD17B10/MRPP2 acting as a non-catalytic subunit . The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme . Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
Swiss Prot
Q99714 Human, O70351 Rat
Cellular Locus
Mitochondrion.
Host
Rabbit
Species Reactivity
Human,Rat
Immunogen
Recombinant protein within human ERAB aa 1-200.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IHC-P
Positive Control
A549 cell lysate, MCF-7 cell lysate, rat kidney tissue, human tonsil tissue, human liver tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000;IHC-P: 1:100-1:500
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
27 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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