Histone H3 (acetyl K27) Rabbit Polyclonal Antibody
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
Histone H3 H3K27ac
Swiss Prot
P68431 Human, P84243 Human, Q16695 Human, Q71DI3 Human, Q6NXT2 Human
Cellular Locus
Chromosome, Nucleosome core, Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthetic peptide within human Histone H3 (acetyl K27) aa 1-50.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IHC-P, ChIP
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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