DAP12 Recombinant Rabbit Monoclonal Antibody [JG38-70]
Natural killer (NK) cells are regulated by stimulatory and inhibitory signals from a variety of receptors. Three main receptor families are responsible for NK cells recognition of MHC I molecules, including Ly-49, CD94/NKG2 and KIR (killer-cell inhibitory receptor). DAP12 is a phosphoprotein that is involved in the activation of NK cells. This protein interacts with membrane glycoproteins of the KIR family, resulting in cellular activation. DAP12 also binds to CD94/NKG2C, an activating NK cell receptor belonging to the C-type lectin superfamily. Additional proteins that bind to DAP12 include Ly-49D and Ly-49H, which associate with DAP12 in the plasma membrane. Phosphorylated DAP12 binds to ZAP-70 and Syk, suggesting that the activation pathway may be similar to that of the T and B cell antigen receptors.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
KAR-associated protein, TYROBP, DAP12, KARAP
Swiss Prot
O43914 Human
Cellular Locus
Membrane.
Host
Rabbit
Species Reactivity
Human
Immunogen
Recombinant protein within Human DAP12 aa 1-113 / 113.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, FC
Positive Control
THP-1 cell lysates, human lung carcinoma tissue, K562.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
12 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
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