PRMT1 Recombinant Rabbit Monoclonal Antibody [JA11-17]
A class of proteins termed type 1 protein arginine N-methyltransferase (PRMT) enzymes contribute to posttranslational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties and subcellular localization. PRMT1, the predominant form in mammalian cells, is located in the nucleus, while PRMT3 is present in the cytoplasm. At the carboxy-terminus, interleukin enhancer-binding factor 3 (ILF3) binds PRMT1, thereby regulating PRMT1 activitiy. Alternative mRNA splicing of the PRMT gene results in three isoforms of PRMT1 that differ in their amino-terminus regions. All three splice variants of PRMT1 are enzymatically active. PRMT3 recognizes and binds to RNA-associated substrates with a zinc-finger domain in its amino-terminus. The zinc-liganded form of this domain is required for the enzyme to recognize RNA-associated substrates.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
PRMT1
Swiss Prot
Q99873 Human, Q9JIF0 Mouse, Q63009 Rat
Cellular Locus
Nucleus. Cytoplasm.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human PRMT1 aa 12-61 / 371.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P
Positive Control
D3, NIH/3T3, Hela, HepG2, SW480, human breast carcinoma tissue, mouse liver tissue, mouse colon tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
42 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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