Rac1-2-3 Recombinant Rabbit Monoclonal Antibody [JM11-29]
Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
p21-Rac1, p21-Rac2, p21-Rac3
Swiss Prot
P63000 Human, P15153 Human, P60763 Human, P63001 Mouse, Q6RUV5 Rat
Cellular Locus
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant full length protein of Human RAC1 aa 1-192 / 192.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P
Positive Control
MCF-7 cell lysate, PC-12 cell lysate, 293T, Hela, NIH/3T3, human spleen tissue, mouse brain tissue, rat brain tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:200 ;IHC-P: 1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 21 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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