PABPN1 Recombinant Rabbit Monoclonal Antibody [JM11-28]
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly (A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly (A) polymerase (PAPOLA) conferring processivity on the poly (A) tail elongation reaction and controls also the poly (A) tail length. Increases the affinity of poly (A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly (A) and to poly (G) with high affinity. May protect the poly (A) tail from degradation.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
PABP-2, Poly (A)-binding protein 2, PABII, PABPN1
Swiss Prot
Q86U42 Human, Q8CCS6 Mouse
Cellular Locus
Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human PABPN1 aa 4-51 / 306.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Positive Control
MCF-7 cell lysate, SH-SY5Y cell lysate, rat skin tissue lysate, AGS, SW480, mouse testis tissue, Hela.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 33 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Lentiviral mouse Prok2 shRNA (UAS) - Lentiviral mouse Prok2 shRNA (UAS) (100)