CaMKII alpha Recombinant Rabbit Monoclonal Antibody [JM11-07]
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
CaM kinase II subunit alpha, CaMK-II subunit alpha
Swiss Prot
Q9UQM7 Human, P11798 Mouse, P11275 Rat
Cellular Locus
Cell junction, Cell projection, Synapse.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human CaMKII alpha aa 37-71 / 478.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IP, FC, IHC-P, IF-Tissue, IF-Cell, IHC-Fr
Positive Control
Mouse brain tissue, rat brain tissue lysates, rat testis tissue, mouse cerebellum tissue, rat brain tissue, Neuro-2a.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1:10-1:50 ;IF-Tissue: 1:50 ;IF-Cell: 1:100 ;IHC-Fr: 1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 54 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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