CYP2E1 Recombinant Rabbit Monoclonal Antibody [JM10-85]
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) .Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).
Product Specifications
Product Name Alternative
Abbreviation
Cytochrome P450 2E1, CYP2E1, CYP2E
Swiss Prot
P05181 Human, Q05421 Mouse, P05182 Rat
Cellular Locus
Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human CYP2E1 aa 71-114 / 439.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Tissue, IHC-P
Positive Control
Human liver tissue lysates, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:5,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 57 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
CAS Number
9000-83-3
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