HRP Conjugated GFP Recombinant Rabbit Monoclonal Antibody [JF51-05]
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
GFP antibody Green fluorescent protein antibody yfp antibody
Swiss Prot
P42212 Aequorea victoria
Host
Rabbit
Species Reactivity
Universal
Immunogen
Recombinant full length protein of Aequorea victoria GFP.
Isotype
IgG
Conjugation
HRP-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB
Positive Control
K-562 transfected with GFP cell lysates.
Concentration
1 mg/mL
Dilution
WB: 1:10,000-1:20,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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