Argonaute 2 Recombinant Rabbit Monoclonal Antibody [JF0992]
Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Argonaute2, hAgo2, eIF-2C 2, eIF2C 2, PPD, AGO2, EIF2C2
Swiss Prot
Q9UKV8 Human, Q8CJG0 Mouse, Q9QZ81 Rat
Cellular Locus
Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human Argonaute 2 aa 361-404 / 859.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Positive Control
HCT 116 cell lysate, HeLa cell lysate, HCT 116, RAW264.7, C6, mouse kidney tissue, mouse stomach tissue, human liver tissue.
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:1,000-1:4,000 ;FC: 1:50-1:100 ;IP: 1-2μg/sample
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 97 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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