Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07]
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Hist1h3a, Hist1h3b
Swiss Prot
P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P68433 Mouse, P84228 Mouse, Q6LED0 Rat
Cellular Locus
Nucleus, Chromosome.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within human Histone H3 aa 85-136/136.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, ChIP, IP
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:20,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:100 ;IHC-P: 1:5,000-1:10,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;IP: 1-2μg/sample
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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