Phospho-B Raf (T401) Recombinant Rabbit Monoclonal Antibody [JJ08-72]
BRAF is a human gene that encodes a protein called B-Raf. The gene is also referred to as proto-oncogene B-Raf and v-Raf murine sarcoma viral oncogene homolog B, while the protein is more formally known as serine/threonine-protein kinase B-Raf. B-Raf is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The B-Raf protein is involved in sending signals inside cells which are involved in directing cell growth. Certain other inherited BRAF mutations cause birth defects. Drugs that treat cancers driven by BRAF mutations have been developed. Two of these drugs, vemurafenib and dabrafenib are approved by FDA for treatment of late-stage melanoma.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
BRAF
Swiss Prot
P15056 Human, P28028 Mouse,
Cellular Locus
Nucleus, Cytoplasm, Cell membrane.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr401 of Human B Raf aa 371-420 / 766.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IP
Positive Control
Mouse testis tissue lysates, PC-12 cell lysates, PC-3M, MCF-7, PC-12.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 84 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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