14-3-3 epsilon Recombinant Rabbit Monoclonal Antibody [JJ08-40]
14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer, and microdeletions associated with Miller–Dieker syndrome.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
14-3-3E, YWHAE
Swiss Prot
P62258 Human, P62259 Mouse, P62260 Rat
Cellular Locus
Nucleus, Cytoplasm, Melanosome.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within human 14-3-3 epsilon aa 51-100.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Positive Control
293T cell lysate, SH-SY5Y cell lysate, Hela, SH-SY5Y, NIH/3T3, mouse placenta tissue, human colon carcinoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:10-1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 29 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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