Phospho-Raf1 (S43) Recombinant Rabbit Monoclonal Antibody [SD087-05]
Selected Variant
ET1612-75
Size: 100 µL
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
cRaf, RAF1
Swiss Prot
P04049 Human, Q99N57 Mouse, P11345 Rat
Cellular Locus
Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser43 of Human Raf1 aa 20-69 / 648.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB
Positive Control
NIH/3T3 cell lysate, 293T cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
73 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Price
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