Phospho-Raf1 (S43) Recombinant Rabbit Monoclonal Antibody [SD087-05]
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
cRaf, RAF1
Swiss Prot
P04049 Human, Q99N57 Mouse, P11345 Rat
Cellular Locus
Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser43 of Human Raf1 aa 20-69 / 648.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB
Positive Control
NIH/3T3 cell lysate, 293T cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
73 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Complement C4d (C4D204), CF405S conjugate, 0.1mg/mL
Cytokeratin, HMW (AE-3), CF647 conjugate, 0.1mg/mL
MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) (VU-11D1), CF568 conjugate, 0.1mg/mL
Cytokeratin, LMW (AE-1), CF594 conjugate, 0.1mg/mL
