Phospho-AMPK alpha 1 (S496) Recombinant Rabbit Monoclonal Antibody [SD0810]
AMPK (5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPKα1 (5'-AMP-activated protein kinase catalytic subunit alpha-1), also known as PRKAA1, is a 559 amino acid protein that belongs to the CAMK Ser/Thr protein kinase family and protein kinase superfamily. Highly phosphorylated, AMPKα1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 5p13.1.
Product Specifications
Product Name Alternative
Abbreviation
AMPK subunit alpha-1, ACACA kinase, HMGCR kinase, PRKAA1
Swiss Prot
Q13131 Human, Q5EG47 Mouse
Cellular Locus
Nucleus, Cytoplasm.
Host
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser496 of Human AMPK alpha 1 aa 471-520 / 559.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IP, IHC-Fr
Positive Control
SiHa cell lysate, HUVEC cell lysate, K-562 cell lysate, mouse hippocampus tissue, mouse cerebral cortex tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000 ;IP: Use at an assay dependent concentration. ;IHC-Fr: 1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 64 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
CAS Number
9000-83-3
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