Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1]
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
MAD homolog 2, Mothers against DPP homolog 2, hMAD-2, SMAD 2, Smad2, hSMAD2
Swiss Prot
Q15796 Human, Q62432 Mouse, O70436 Rat
Cellular Locus
Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser250 of Human Smad2 aa 221-270 / 467.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, FC, IP
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:5,000 ;FC: 1:50-1:100 ;IP: 1-2μg/sample
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 52 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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