Phospho-Hsp27 (S82) Recombinant Rabbit Monoclonal Antibody [SN06-73]
HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
HspB1, HSP 27, SRP27, HSPB1
Swiss Prot
P04792 Human, P14602 Mouse, P42930 Rat
Cellular Locus
Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser82 of Human Hsp27 aa 50-99 / 205.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IP
Positive Control
Human skeletal muscle tissue lysate, MCF-7 cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000 ;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 23 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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