CD14 Recombinant Rabbit Monoclonal Antibody [SC69-02]
Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
CD14
Swiss Prot
P08571 Human, P10810 Mouse
Cellular Locus
Cell membrane, Secreted, Golgi apparatus, Membrane raft.
Host
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthetic peptide within Human CD14 aa 310-335 / 375.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IHC-P, mIHC, FC
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:200-1:800 ;mIHC: 1:800-1:1,000 ;FC: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 40 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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