PKR Recombinant Rabbit Monoclonal Antibody [SC06-37]
An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the a subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2a on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA mediated autophosphorylation.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
eIF-2A protein kinase 2, PKR, Protein kinase R, EIF2AK2
Swiss Prot
P19525 Human
Cellular Locus
Nucleus, Cytoplasm, perinuclear region.
Host
Rabbit
Species Reactivity
Human
Immunogen
Synthetic peptide within human PKR aa 500-540.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Positive Control
MCF-7 cell lysate, Hela cell lysate, MCF-7, human kidney tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:5,000;IF-Cell: 1:50-1:100;IF-Tissue: 1:50-1:100;IHC-P: 1:50-1:100;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 62 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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