p95 / NBS1 Recombinant Rabbit Monoclonal Antibody [SC05-68]
DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the double-strand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Dmc1 and nibrin. Dmc1 is specifically involved in meiotic recombination. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
NBN
Swiss Prot
O60934 Human, Q9R207 Mouse, Q9JIL9 Rat
Cellular Locus
Nucleus, Chromosome.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within human p95 NBS1 aa 680-730.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IP, FC, IHC-P
Positive Control
HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, Hela, A431, human large intestine tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;FC: 1:50-1:100 ;IHC-P:1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 85 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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