Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427]
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Hist1h3a, Hist1h3b,H3R2me1
Swiss Prot
P68431 Human, P84243 Human, Q6NXT2 Human, Q71DI3 Human, Q16695 Human, P84228 Mouse, P68433 Mouse, Q6LED0 Rat
Cellular Locus
Nucleus, Chromosome.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within human Histone H3 aa 1-49/136.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, ChIP
Positive Control
HepG2, NIH/3T3, PC-3M, human skin tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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