AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [ST48-09]

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Product Specifications

CAS Number

9000-83-3

Product Name Alternative

AKT antibody AKT1 antibody AKT1 kinase antibody AKT1m antibody AKT2 antibody AKT2 kinase antibody Akt3 antibody AKT3_HUMAN antibody CAKT antibody CWS6 antibody DKFZp434N0250 antibody HIHGHH antibody kinase Akt1 antibody MGC99656 antibody MPPH antibody Murine thymoma viral (v-akt) homolog 2 antibody PKB ALPHA antibody PKB antibody PKB beta antibody PKB gamma antibody PKB-GAMMA antibody PKB/Akt antibody PKBALPHA antibody PKBB antibody PKBBETA antibody PKBG antibody PKBGAMMA antibody PRKBA antibody PRKBB antibody PRKBG antibody Protein kinase Akt 2 antibody Protein kinase Akt-3 antibody Protein kinase B alpha antibody Protein kinase B antibody Protein kinase B beta antibody Protein kinase B gamma antibody Proto oncogene c Akt antibody RAC ALPHA antibody RAC alpha serine/threonine protein kinase antibody RAC antibody RAC BETA antibody RAC beta serine/threonine protein kinase antibody RAC PK alpha antibody RAC PK beta antibody rac protein kinase alpha antibody rac protein kinase beta antibody RAC-gamma antibody RAC-gamma serine/threonine-protein kinase antibody RAC-PK-gamma antibody RACALPHA antibody RACalpha serine/threonine kinase antibody RACBETA antibody RACgamma antibody RACgamma serine/threonine protein kinase antibody RACPKgamma antibody serine threonine protein kinase antibody STK-2 antibody STK2 antibody thymoma viral proto oncogene 1 antibody thymoma viral proto oncogene antibody V akt murine thymoma viral oncogene homolog 1 antibody V akt murine thymoma viral oncogene homolog 2 antibody V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody V akt murine thymoma viral oncogene homolog 3 antibody vakt murine thymoma viral oncogene homolog 1 antibody vakt murine thymoma viral oncogene homolog 2 antibody vakt murine thymoma viral oncogene homolog 3 antibody

Abbreviation

PKB, PKB alpha, AKT1, PKB beta, AKT2, PKB gamma, AKT3

Swiss Prot

P31749 Human, P31751 Human, Q9Y243 Human, P31750 Mouse, Q60823 Mouse, Q9WUA6 Mouse, P47196 Rat, P47197 Rat, Q63484 Rat

Cellular Locus

Cell membrane, Cytoplasm, Membrane, Nucleus.

Host

Rabbit

Species Reactivity

Human,Mouse,Rat,Monkey

Immunogen

Recombinant protein within human AKT3 aa 300-479.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, IHC-Fr

Positive Control

MCF7 cell lysate, U-2 OS cell lysate, Jurkat cell lysate, C6 cell lysate, mouse heart tissue lysate, mouse testis tissue lysate, rat heart tissue lysate, rat testis tissue lysate, MCF7, RAW264.7, C6, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue, rat brain tissue, rat lung tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.